FORMULASI MIKROEMULSI EKSTRAK FULI UNTUK MENGHAMBAT FOTOOKSIDASI VITAMIN C DALAM SISTEM AQUEOUS

• Main Authors: , HASBULLAH, , Prof. Dr. Ir. Sri Raharjo, M.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2011
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/90225/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=52403
• Institution: Universitas Gadjah Mada

The objective of this research was to obtain stable microemulsion formula containing mace extract which effectivelly inhibits the photooxidation of vitamin C in aqueous system. Mace extract was obtained from extraction process by using organic solvent. Total phenolic content (TPC) and β-carotene content of the mace extract was measured. Antioxidative activitiy of mace extract was determined by DPPH and linoleic method. Microemulsion o/w acts as delivery system for mace extract was prepared by using virgin coconut oil (VCO), aquadest and combination of three nonionic surfactants. The stability of the obtained microemulsion formula was tested against room temperature storage for 8 weeks under light exposure and dark condition, centrifugation 4000 rpm for 15 minutes, the heating in oven at 105oC for 5 hours. Microemulsion containing mace extract prepared by adding mace extract as much as 500 ppm and 750 ppm to the o/w microemulsion and its stability was tested against room temprature storage for 4 weeks under light exposure and dark condition, centrifugation 4000 g for 15 hours and heating in oven at 105oC for 5 hours both with or without dilution 1:9 with citrate buffer pH 3.1. Antiphotooxidative effect of microemulsion containing mace extract was determined based on the rate of vitamin C degradation in two types of aqueous systems: beverage model system and apple juice in which each added with 20 ppm of erytrosine as sesitizer and 100 ppm of vitamin C. The rate of vitamin C photooxidation was determined by observing the amount of vitamin C in the system at the starting time, following by 2, 4, 6 and 8 hours after that. The result shows that the added microemulsion containing mace extract decreased the rate of vitamin C degradation in both aqueous systems which mean microemulsion containing mace extract inhibited photooxidation of vitamin C in aqueous system.