High-throughput cloning of Campylobacter jejuni ORFs by in vivo recombination in Escherichia coli

• المؤلفون الرئيسيون: Jodi R. Parrish, Thawornchai Limjindaporn, Julie A. Hines, Jiayou Liu, Guozhen Liu, Russell L. Finley
• مؤلفون آخرون: Wayne State University School of Medicine
• التنسيق: مقال
• منشور في: 2018
• الموضوعات: Biochemistry, Genetics and Molecular Biology; Chemistry
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/21191
• المؤسسة: Mahidol University

A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. T...