Screening for Methanol-Utilizing Yeasts Having Methyl formate-producing activity = Seleksi Khamis pengguna Metanol yang mampu menghasilkan Metil Format
Methanol-utilizing yeasts were screened for methyl formate- producing activity. Eleven strains out of twenty strains of yeasts tested were found to have the activity. Pichia methanolica showed the highest activity, followed by Candida sp. N-16, Candida sp. 1 -B, Pichia pinus, and Candida sp. 25-A, r...
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Format: | Article NonPeerReviewed |
Published: |
[Yogyakarta] : Universitas Gadjah Mada
1991
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Subjects: | |
Online Access: | https://repository.ugm.ac.id/17324/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=76 |
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Summary: | Methanol-utilizing yeasts were screened for methyl formate- producing activity. Eleven strains
out of twenty strains of yeasts tested were found to have the activity. Pichia methanolica
showed the highest activity, followed by Candida sp. N-16, Candida sp. 1 -B, Pichia pinus, and
Candida sp. 25-A, respectively. Methyl formate was found to accumulate in the cultural media
of those strains during the course of the their growth in methanol- containing media. Along
with the methyl formate-producing activity it wasfoundthat resting cells also had a hydrolyzing
|activity against methyl formate. Cultivation and reaction conditions were optimized with
respect to methyl formate production. The methyl formate-producing activity was strongly
induced by methanol but weakly induced by glycerol. On the other hand, the methyl
formate-producing activity was not induced by ethanol and glucose. However, methyl formate-
hydrolyzing activity was induced by methanol, glycerol, glucose, and ethanol although with
different intensities.
Key words: methanol yeast - methyl formate |
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