Screening for Methanol-Utilizing Yeasts Having Methyl formate-producing activity = Seleksi Khamis pengguna Metanol yang mampu menghasilkan Metil Format

Methanol-utilizing yeasts were screened for methyl formate- producing activity. Eleven strains out of twenty strains of yeasts tested were found to have the activity. Pichia methanolica showed the highest activity, followed by Candida sp. N-16, Candida sp. 1 -B, Pichia pinus, and Candida sp. 25-A, r...

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Bibliographic Details
Main Author: Perpustakaan UGM, i-lib
Format: Article NonPeerReviewed
Published: [Yogyakarta] : Universitas Gadjah Mada 1991
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Online Access:https://repository.ugm.ac.id/17324/
http://i-lib.ugm.ac.id/jurnal/download.php?dataId=76
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Summary:Methanol-utilizing yeasts were screened for methyl formate- producing activity. Eleven strains out of twenty strains of yeasts tested were found to have the activity. Pichia methanolica showed the highest activity, followed by Candida sp. N-16, Candida sp. 1 -B, Pichia pinus, and Candida sp. 25-A, respectively. Methyl formate was found to accumulate in the cultural media of those strains during the course of the their growth in methanol- containing media. Along with the methyl formate-producing activity it wasfoundthat resting cells also had a hydrolyzing |activity against methyl formate. Cultivation and reaction conditions were optimized with respect to methyl formate production. The methyl formate-producing activity was strongly induced by methanol but weakly induced by glycerol. On the other hand, the methyl formate-producing activity was not induced by ethanol and glucose. However, methyl formate- hydrolyzing activity was induced by methanol, glycerol, glucose, and ethanol although with different intensities. Key words: methanol yeast - methyl formate