Screening for Methanol-Utilizing Yeasts Having Methyl formate-producing activity = Seleksi Khamis pengguna Metanol yang mampu menghasilkan Metil Format

• Main Author: Perpustakaan UGM, i-lib
• Format: Article NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 1991
• Subjects: Jurnal i-lib UGM
• Online Access: https://repository.ugm.ac.id/17324/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=76
• Institution: Universitas Gadjah Mada

Methanol-utilizing yeasts were screened for methyl formate- producing activity. Eleven strains out of twenty strains of yeasts tested were found to have the activity. Pichia methanolica showed the highest activity, followed by Candida sp. N-16, Candida sp. 1 -B, Pichia pinus, and Candida sp. 25-A, respectively. Methyl formate was found to accumulate in the cultural media of those strains during the course of the their growth in methanol- containing media. Along with the methyl formate-producing activity it wasfoundthat resting cells also had a hydrolyzing |activity against methyl formate. Cultivation and reaction conditions were optimized with respect to methyl formate production. The methyl formate-producing activity was strongly induced by methanol but weakly induced by glycerol. On the other hand, the methyl formate-producing activity was not induced by ethanol and glucose. However, methyl formate- hydrolyzing activity was induced by methanol, glycerol, glucose, and ethanol although with different intensities. Key words: methanol yeast - methyl formate