The Potency of Bioactive Peptide of Native Chicken Leg as an Anti-hipertency Agent
Hypertension is the most common serious chronic health problem in adults and carries a high risk factor for arteriosclerosis, strokes, and end-stage renal disease. The objective of this research was to investigate the potency of chicken leg protein for the inhibition of angiotensin converting enzyme...
محفوظ في:
| المؤلفون الرئيسيون: | , , , , |
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| التنسيق: | Conference or Workshop Item NonPeerReviewed |
| اللغة: | English |
| منشور في: |
2014
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://repository.ugm.ac.id/139188/1/The_16th_AAAP_Jamhari%20-%2012%20%28Poster%29.pdf https://repository.ugm.ac.id/139188/ http://aaap2014.ugm.ac.id |
| الوسوم: |
إضافة وسم
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| المؤسسة: | Universitas Gadjah Mada |
| اللغة: | English |
| الملخص: | Hypertension is the most common serious chronic health problem in adults and carries a high risk factor for arteriosclerosis, strokes, and end-stage renal disease. The objective of this research was to investigate the potency of chicken leg protein for the inhibition of angiotensin converting enzyme (ACE) activity. Protein of chicken leg expected as a source of bioactive peptides that can be used as antihypertensive agent. The stages of research were preparation chicken leg protein, digestion of chicken leg protein by pepsin and trypsin, determination of ACE inhibitory activity, and purification of ACE inhibitory peptides. Digestion process was measured by soluble protein cocentration and molecular weight profile of native protein fraction as well as after pepsin and trypsin digestion. The result showed concentration of soluble protein using biuret test was 2.54 ± 0.62 mg/ml while after pepsin and trypsin were 43.65 ± 0.89 and 37.78 ± 0.84 mg/ml, respectively. Molecular weight (kDa) of chicken leg consecutive was 84.45; 76.31; 53.50; 43.67, and 41.52 kDa, after pepsin digestion was 43.67, 30.62; and 22.02 kDa and after trypsin digestion was 33.89; 23.76; and 14.31 kDa. Inhibitory activity of chicken leg protein on ACE was 65.24% and the IC50 value was 2.98 mg/ml. ACE Inhibitory activity of chicken leg protein hydrolyzed after purification using SEP-PAK Plus Cartridge C-18 increased to 70.05 % and the IC50 value was 2.03 mg/ml.In conclusion, native chicken leg has a high potential to be utilized and produced ACE inhibitory peptides.
Key Words: Chicken leg, Bioactive peptides, Hypertension, ACE inhibition |
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