UJI AKTIVITAS ANTIJAMUR EKSTRAK METANOL PELEPAH BATANG PISANG AMBON (Musa acuminata) TERHADAP Achlya sp. SECARA IN VITRO
Infectious diseases caused by Oomycetes become one of obstacle in aquaculture. Based on interview with some fish farmers, banana pseudostem could be used to overcome saprolegniasis. Saprolegniasis could be caused by Saprolegnia sp or Achlya sp infection. The purpose of this study were to determi...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/131936/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72447 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Infectious diseases caused by Oomycetes become one of obstacle in
aquaculture. Based on interview with some fish farmers, banana pseudostem could be
used to overcome saprolegniasis. Saprolegniasis could be caused by Saprolegnia sp or
Achlya sp infection. The purpose of this study were to determine the antifungal activity
of metanol extract of ambon banana (Musa acuminata) pseudostem and to identify it�s
bioactive compounds. Ambon banana pseudostem was collected fom Bantul while the
infected gouramy was collected from Pingit market, Yogyakarta. The banana
pseudostem was chopped, dried,and extracted. Extraction was done using maceration
method with methanol as solvent. The fungi from gouramy was inoculated on potato
dextrose agar (PDA) and then was incubated. Identification was done by observing it�s
sporangium morphology. Fungi purificaton was carried out on PDA medium
supplemented by ciprofloxacin 1 mg/ml. The antifungal activity assay was done using
agar diffusion and dilution method. The phytochemical screening was done using 96
microwell plate and using thin layer chromatography (TLC) plates. In addition,
saponin and tannin screening was also performed. The result showed the fungi which
infected fish is Achlya sp. Methanol extract of ambon banana did not posses antifungal
activity against Achlya sp. Phytochemistry screening showed that methanol extract
ambon banana pseudostem contain terpen and saponin. Further studies was needed to
determine antifungal activity from different banana species and to determine the effect
of banana pseudostem application on water quality. |
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