การสังเคราะห์อนุภาคนาโนทองแดงสำหรับตรวจวัดโปรตีนในอาหาร

• المؤلف الرئيسي: พศวีร์ พรพินิจสุวรรณ
• مؤلفون آخرون: นำพล อินสิน
• التنسيق: Senior Project
• اللغة: Thai
• منشور في: จุฬาลงกรณ์มหาวิทยาลัย 2016
• الموضوعات: อนุภาคนาโน; โปรตีน > การวิเคราะห์; Nanoparticles; Copper
• الوصول للمادة أونلاين: https://digiverse.chula.ac.th/Info/item/dc:10005
• المؤسسة: Chulalongkorn University

Copper nanoparticles were synthesized by reduction method, using copper(II) sulfate, tri-sodium citrate and hydrazine for copper precursor, stabilizer and reducing agent, respectively, in molar ratio of 1:8:6. Copper nanoparticles were characterized using X-ray Diffraction (XRD), Transmission Electron Microscope (TEM) and UV-vis Spectroscopy, and it was found that copper nanoparticles have diameter of around 30 nm. Copper nanoparticles were then used for determining protein content of standard solution (Bovine Serum Albumin; BSA) and sample solution (milk) by UV-vis Spectroscopy. Protein content was detected in a linear range of 0-16 μg/mL for both solutions. The limit of detection is 3 x 10⁻⁵ mg/mL for BSA solution and 8 x 10⁻⁵ mg/mL for milk solution. The effect of concentration of copper nanoparticles on protein content measurement was studied. The more copper nanoparticles, the wider linear range for protein content measurement.