Ammonium sulfate improves sensitivity and avoids false negatives of polymerase chain reaction (PCR) for scale drop disease virus (SDDV) detection

• المؤلفون الرئيسيون: Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin
• مؤلفون آخرون: Suan Sunandha Rajabhat University
• التنسيق: مقال
• منشور في: 2022
• الموضوعات: Agricultural and Biological Sciences
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/75698

Accuracy is crucial for polymerase chain reaction (PCR) diagnostic laboratories. We noticed inconsistent PCR results or false negatives that sometimes occurred when changing commercial brand of Taq DNA polymerase enzyme. Here, we tested 6 primer sets specific for 3 important aquaculture viruses (sca...