Ammonium sulfate improves sensitivity and avoids false negatives of polymerase chain reaction (PCR) for scale drop disease virus (SDDV) detection
Accuracy is crucial for polymerase chain reaction (PCR) diagnostic laboratories. We noticed inconsistent PCR results or false negatives that sometimes occurred when changing commercial brand of Taq DNA polymerase enzyme. Here, we tested 6 primer sets specific for 3 important aquaculture viruses (sca...
Saved in:
Main Authors: | Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin |
---|---|
Other Authors: | Suan Sunandha Rajabhat University |
Format: | Article |
Published: |
2022
|
Subjects: | |
Online Access: | https://repository.li.mahidol.ac.th/handle/123456789/75698 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
A validated semi-nested PCR for rapid detection of scale drop disease virus (SDDV) in Asian sea bass (Lates calcarifer)
by: Onanong Charoenwai, et al.
Published: (2020) -
Draft genome sequence of scale drop disease virus (SDDV) retrieved from metagenomic investigation of infected barramundi, Lates calcarifer (Bloch, 1790)
by: Pattanapon Kayansamruaj, et al.
Published: (2020) -
A sensitive and specific SYBR Green-based qPCR assay for detecting scale drop disease virus (SDDV) in Asian sea bass
by: Sukhontip Sriisan, et al.
Published: (2020) -
CRISPR-based platform for rapid, sensitive and field-deployable detection of scale drop disease virus in Asian sea bass (Lates calcarifer)
by: Thanwarat Sukonta, et al.
Published: (2022) -
False rumours of disease outbreaks caused by infectious myonecrosis virus (IMNV) in the whiteleg shrimp in Asia
by: Saengchan Senapin, et al.
Published: (2018)