Ammonium sulfate improves sensitivity and avoids false negatives of polymerase chain reaction (PCR) for scale drop disease virus (SDDV) detection

• Main Authors: Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin
• 其他作者: Suan Sunandha Rajabhat University
• 格式: Article
• 出版: 2022
• 主題: Agricultural and Biological Sciences
• 在線閱讀: https://repository.li.mahidol.ac.th/handle/123456789/75698

Accuracy is crucial for polymerase chain reaction (PCR) diagnostic laboratories. We noticed inconsistent PCR results or false negatives that sometimes occurred when changing commercial brand of Taq DNA polymerase enzyme. Here, we tested 6 primer sets specific for 3 important aquaculture viruses (sca...