Ammonium sulfate improves sensitivity and avoids false negatives of polymerase chain reaction (PCR) for scale drop disease virus (SDDV) detection

• Main Authors: Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin
• Other Authors: Suan Sunandha Rajabhat University
• Format: Article
• Published: 2022
• Subjects: Agricultural and Biological Sciences
• Online Access: https://repository.li.mahidol.ac.th/handle/123456789/75698
• Institution: Mahidol University

Accuracy is crucial for polymerase chain reaction (PCR) diagnostic laboratories. We noticed inconsistent PCR results or false negatives that sometimes occurred when changing commercial brand of Taq DNA polymerase enzyme. Here, we tested 6 primer sets specific for 3 important aquaculture viruses (sca...