Effective enhancement of Pseudomonas stutzeri D-phenylglycine aminotransferase functional expression in Pichia pastoris by co-expressing Escherichia coli GroEL-GroES

• Main Authors: Kanidtha Jariyachawalid, Poramaet Laowanapiban, Vithaya Meevootisom, Suthep Wiyakrutta
• 其他作者: Mahidol University. Faculty of Science. Department of Microbiology
• 格式: Article
• 語言: English
• 出版: 2017
• 主題: Open Access article; D-phenylglycine aminotransferase (D-PhgAT); Pseudomonas stutzeri ST-201; Pichia pastoris; Escherichia coli GroEL-GroES
• 在線閱讀: https://repository.li.mahidol.ac.th/handle/123456789/2727
• 機構: Mahidol University

Background: D-phenylglycine aminotransferase (D-PhgAT) of Pseudomonas stutzeri ST-201 catalyzes the reversible stereo-inverting transamination potentially useful in the application for synthesis of D-phenylglycine and D-4-hydroxyphenylglycine using L-glutamate as a low cost amino donor substrate i...