A real-time RT-PCR method for the universal detection and identification of flaviviruses

• المؤلفون الرئيسيون: G. Moureau, S. Temmam, J. P. Gonzalez, R. N. Charrel, G. Grard, X. De Lamballerie
• مؤلفون آخرون: Faculte de Medecine de Marseille Universite de la Mediterranee
• التنسيق: مقال
• منشور في: 2018
• الموضوعات: Immunology and Microbiology; Medicine
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/24476

Here we describe an optimized molecular protocol for the universal detection and identification of flaviviruses. It combines the convenient real-time polymerase chain reaction (PCR) format with a broad spectrum of flavivirus detection. This assay, based on the amplification of a 269-272 nt (dependin...