Spectrophotometric enzymatic cycling method using L-glutamate dehydrogenase and D-phenylglycine aminotransferase for determination of L-glutamate in foods

• المؤلفون الرئيسيون: Wanida Khampha, Vithaya Meevootisom, Suthep Wiyakrutta
• مؤلفون آخرون: Mahidol University
• التنسيق: مقال
• منشور في: 2018
• الموضوعات: Biochemistry, Genetics and Molecular Biology; Chemistry; Environmental Science
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/21166

This report describes a new spectrophotometric method capable of determining low levels of L-glutamate. The assay is based on substrate cycling between L-glutamate dehydrogenase (GlDH) and the novel enzyme D-phenylglycine aminotransferase (D-PhgAT). In this system, GlDH converts L-glutamate to 2-oxo...