Phytase production from Pseudomonas putida harbouring Escherichia coli appA

• المؤلفون الرئيسيون: Saovanee Dharmsthiti, Sarunya Chalermpornpaisarn, Mardvipa Kiatiyajarn, Amaraporn Chanpokapaiboon, Yaikaew Klongsithidej, Jetnapha Techawiparut
• مؤلفون آخرون: Mahidol University
• التنسيق: مقال
• منشور في: 2018
• الموضوعات: Biochemistry, Genetics and Molecular Biology; Chemistry; Engineering
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/16377

An Escherichia coli acid phosphatase gene with phytase activity was cloned into pBBRT plasmid and transformed into Pseudomonas putida. A complex medium was developed for phytase production from this recombinant strain. After cultivation at 30°C for 48 h crude phytase was isolated and characterized....