A prospective assessment of the accuracy of commercial IgM ELISAs in diagnosis of Japanese encephalitis virus infections in patients with suspected central nervous system infections in Laos

• Main Authors: Catrin E. Moore, Stuart D. Blacksell, Thaksinaporn Taojaikong, Richard G. Jarman, Robert V. Gibbons, Sue J. Lee, Vilada Chansamouth, Soulignasack Thongpaseuth, Mayfong Mayxay, Paul N. Newton
• 其他作者: Mahosot Hospital
• 格式: Article
• 出版: 2018
• 主題: Immunology and Microbiology; Medicine
• 在線閱讀: https://repository.li.mahidol.ac.th/handle/123456789/14297
• 機構: Mahidol University

Japanese encephalitis virus (JEV) is a major cause of encephalitis in Asia. We estimated the diagnostic accuracy of two anti-JEV immunoglobulin M (IgM) enzyme-linked immunosorbent assays (ELISAs) (Panbio and XCyton JEVCheX) compared with a reference standard (AFRIMS JEV MAC ELISA) in a prospective study of the causes of central nervous system infections in Laos. Cerebrospinal fluid (CSF; 515 patients) and serum samples (182 patients) from those admitted to Mahosot Hospital, Vientiane, were tested. The CSF from 14.5% of acute encephalitis syndrome (AES) patients and 10.1% from those with AES and meningitis were positive for anti-JEV IgM in the reference ELISA. The sensitivities for CSF were 65.4%(95%confidence interval [CI] = 51-78) (Xcyton), 69.2% (95%CI = 55-81) (Panb io), however 96.2% (95% CI = 87-100) with Panbio Ravi criteria. Specificities were 89-100%. For admission sera from AES patients, sensitivities and specificities of the Panbio ELISA were 85.7% (95% CI = 42-100%) and 92.9% (95% CI = 83-98%), respectively. Copyright © 2012 by The American Society of Tropical Medicine and Hygiene.