Effective improvement of D-phenylglycine aminotransferase solubility by protein crystal contact engineering

• المؤلفون الرئيسيون: Aiya Chantarasiri, Vithaya Meevootisom, Duangnate Isarangkul, Suthep Wiyakrutta
• مؤلفون آخرون: Mahidol University
• التنسيق: مقال
• منشور في: 2018
• الموضوعات: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology
• الوصول للمادة أونلاين: https://repository.li.mahidol.ac.th/handle/123456789/13645

Structure-guided genetic engineering of D-phenylglycine aminotransferase (D-PhgAT) aimed at increasing protein solubility was attempted. In silico analyses predicted the Asn439 and Gln444 as highly solvent-exposed β-turn residues involved with protein crystal contact (CC) potential candidates for so...