Development of test kit for detection of rifampin resistance in mycobacterium tuberculosis by PCR-reverse line blot hybridization

• المؤلفون الرئيسيون: Somying Tumwasorn, Ajcharaporn Sawatpanich, Nibondh Udomsantisuk, Kamon Kawkitinarong
• مؤلفون آخرون: Chulalongkorn University. Faculty of Medicine
• التنسيق: Technical Report
• اللغة: English
• منشور في: Faculty of Medicine, Chulalongkorn University 2010
• الموضوعات: Rifampin; Drug resistance; Mycobacterium tuberculosis; Tuberculosis
• الوصول للمادة أونلاين: http://cuir.car.chula.ac.th/handle/123456789/13250
• المؤسسة: Chulalongkorn University

A genetic test by PCR-reverse line blot hybridization was developed for rapid detection of mycobacterium tuberculosis and rifampin resistance simultaneously. Duplex PCR targeting IS 6110 and rpoB gene was employed to detect M. tuberculosis and the rpoB fragment with rifampin resistance hot spot regi...