Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for monoclonal antibody expression level and quality in CHO cells

• Main Authors: Bardor, Muriel, Li, Bin, Lee, Jia Juan, Song, Zhiwei, Tong, Yen Wah, Goh, Lin-Tang, Yang, Yuansheng, Ho, Steven C. L.
• 其他作者: School of Chemical and Biomedical Engineering
• 格式: Article
• 語言: English
• 出版: 2013
• 在線閱讀: https://hdl.handle.net/10356/97890; http://hdl.handle.net/10220/13223

Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on monoclonal antibody (mAb) expression level and qu...