An Alternative Method for Evaluating Stabilities of DNA Hairpin Structures

• Main Authors: Zhang, Hao, Ba, Sai, Co Co, Sarajane, Lee, Jasmine Yiqin, Guo, Juanjuan, Ye, Ruijuan, Liang, Zhaoxun, Huang, Dejian, Li, Tianhu
• 其他作者: School of Biological Sciences
• 格式: Article
• 語言: English
• 出版: 2016
• 主題: Oligonucleotides; DNA ligases
• 在線閱讀: https://hdl.handle.net/10356/82904; http://hdl.handle.net/10220/40359

We have developed an alternative methodology for determining the stabilities of DNA hairpin structures based on a specially designed oligonucleotide template and the T4 DNA ligase. This method was systematically tested by evaluation of DNA hairpin stabilities when varying the loop size and the natur...