Analysis of the role played by ligand-induced folding of the cocaine-binding aptamer in the photochrome aptamer switch assay

• المؤلفون الرئيسيون: Shoara, Aron A., Churcher, Zachary R., Steele, Terry W. J., Johnson, Philip E.
• مؤلفون آخرون: School of Materials Science and Engineering
• التنسيق: مقال
• اللغة: English
• منشور في: 2020
• الموضوعات: Engineering::Materials; Biosensors; Aptamer
• الوصول للمادة أونلاين: https://hdl.handle.net/10356/143105
• المؤسسة: Nanyang Technological University

The Photochrome Aptamer Switch Assay (PHASA) relies on ligand binding by an aptamer to alter the local environment of a stilbene compound covalently attached to the 5' end of the aptamer. We used the PHASA with both structure switching and non-structure switching versions of the cocaine-binding...