In vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves

• Main Authors: Mirajuddin, Mirajuddin, Kustono, Kustono, Ismaya, Ismaya, Budiyanto, Agung
• 格式: Conference or Workshop Item NonPeerReviewed
• 語言: English
• 出版: 2010
• 主題: Animal Reproduction
• 在線閱讀: https://repository.ugm.ac.id/137848/1/ISTAP%202010%20%28Ismaya-33%29.pdf; https://repository.ugm.ac.id/137848/
• 機構: Universitas Gadjah Mada

A research on in vitro fresh sperm preparation for maintaining sperm viability at storage temperature of 14°C using tannin supplementation of lamtoro leaves (Leucaena leucocephala) had been carried out in both Physiology and Animal Reproduction Laboratory, Faculty of Animal Science, Universitas Gadjah Mada for fresh sperm preparation and sperm quality evaluation, during March to July of 2009.The experiment was designed using the Complete Random Design consisted of 5 treatments and 5 replications. Variables measured were viability, motility and morphology of the treated fresh sperm stored for 14 days at temperature of 14°C. Based on the analysis of variance, the treatments had significant effect (P<0.05) on viability, motility and morphology of the sperm. In terms of viability, Pl(64.07%±0.45) it was higher than control (P0=26.71%±0.54), but it was not different for P2(57.44%±0.52), P3(49.49%±0.71), and P4 (47.83%±0.61). The progress and very progress motility of spermatozoa were only found in control, and not in others. Non-progressive spermatozoa proportion ofPI, P2, P3 and P4,was found respectively. They were significantly higher than control.For the morphology, P4 had lower normal spermatozoa percentage than P0, PI, P2 and P3, whereas among P0, PI, P2, and P3, there were not different (P>0,05). Key words: fresh sperm preparation, sperm quality, viability, tannin, Lamtoro leaves