Efforts for Ex-situ onservation of Some Cultivars of Clove Syzygium aromaticum (L.) Merrill et. Perry and Its Rhizosphere Microorganisms from Ternate and Saparua, North Maluku

• Main Authors: Retno Peni, Sancayaningsih, A., Indriyanto, E.H., Poentyanti
• Format: Conference or Workshop Item PeerReviewed
• Language: English
• Published: 2009
• Subjects: Ecology
• Online Access: https://repository.ugm.ac.id/136262/1/efforts%20for%20Ex-situ.pdf; https://repository.ugm.ac.id/136262/
• Institution: Universitas Gadjah Mada

Syzygium aromaticum (L.) Merrill et. Perry cv “Afo” is an endemic clove that very productive and may grow for hundred years and almost extinct. It is found in Air Tege-tege, Gamalama, Ternate, North Maluku. While variety of ”Hari-hari” clove flowers seasonal-independently, and it is only found endemic in Saparua, Maluku. Ecologically, germplasm conservation of endemic species deserves both 2 conservations, in vitro plant propagation and conservation of their rhizosphere mutualistic microorganisms. This study aims to conserve both plant and its mutualistic microorganisms of the rhizosphere. Shoots, microspores, and epicotyls from varies maturity cannot be used for explant inisiation directly, due to easily contaminated and browning problems. Shoots from seed rejuvenation of Afo, and Hari-hari were used for explant propagation using MS modified medium. Calli were produced from leave explant of in-vitro seedling of Afo and Hari-hari. There were 5 isolates of phosphate bacteria isolated from soil samples taken from 12 clove varieties’ rhizospheres. Mycorrhizal colonizations were low in test plant and were unstable during the first and second multiplications, except colonization produced by inoculant taken from soil rhizosphere from Radja, Sikotok and Anak-afo that ranged from 20% to 33 %. Key-words : mycorrhiza, clove cv afo, hari-hari, radja, and sikotok