KARAKTERISTIK GELATIN KULIT IKAN TUNA (Thunnus albacares) DAN APLIKASINYA UNTUK NANOENKAPSULASI VITAMIN C
Encapsulation is technique to coat core materials, in form of gas, solid, or liquid, using various wall materials for protection against deterioration. Various substances could be utilizes as wall material, such as polysaccharide, fat and wax, as well as protein, such as gelatin. Gelatin made from T...
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| Format: | Theses and Dissertations NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
2015
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| Online Access: | https://repository.ugm.ac.id/134832/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=77997 |
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| Summary: | Encapsulation is technique to coat core materials, in form of gas, solid, or liquid, using various wall materials for protection against deterioration. Various substances could be utilizes as wall material, such as polysaccharide, fat and wax, as well as protein, such as gelatin. Gelatin made from Tuna (Thunnus albacares) skin was used as wall material to coat vitamin C as core material. This research was an effort to study vitamin C nanoencapsulation using two step desolvation method, to produce particle in size of nanometer (10-1000nm). The objectives were to determine vitamin C concentration and glutaraldehyde volume to produce capsule in nano size as well as to characterize nanocapsule produced. Observed parameters on gelatin were proximate analysis consisted of moisture content, ash content, protein, and fat, and physicochemical analysis consisted of pH, gel strength, viscosity, and melting point. Moisture content of gelatin was 6.02%, ash content was 0.56%, protein and fat content was 82.95% and 0.22%. Acidity degree (pH) of Tuna skin gelatin was 5,96, gel strength was 219,51 bloom, viscosity was 7,1cP, and melting point was 31,7°C. Observed parameters on nanocapsule were nanocapsule staining, solubility, encapsulation efficiency toward vitamin C content, particle morphology using Scanning Electron Microscopy (SEM), and distribution and particle size using Particle Size Analyzer (PSA). The result from particle measurement analysis indicated that all treatment variations able to produce particle in nanometer (10-1000nm). The best treatment was obtained using 2% vitamin C and 200µL glutaraldehyde, indicated by the best morphology and highest encapsulation efficiency. Based on the result, it was concluded that utilization of gelatin from tuna’s skin using two step desolvation method was able to produce nanoparticle with morphology and size similar to those prepared using mammalian gelatin. |
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