PENGARUH TELMISARTAN TERHADAP KADAR TGF-B1 (Transforming Growht Factor-B1) DAN FRAKSI VOLUME KOLAGEN MIOKARDIAL PADA TIKUS PUTIH (Rattus norvegicus) GALUR WISTAR HIPERTENSI DENGAN INDUKSI NATRIUM KLORIDA 8%

• المؤلفون الرئيسيون: , Rachmat Hidayat, , Dr.Med.dr. Widharto Prawirohardjono, Sp.FK
• التنسيق: Theses and Dissertations NonPeerReviewed
• منشور في: [Yogyakarta] : Universitas Gadjah Mada 2014
• الموضوعات: ETD
• الوصول للمادة أونلاين: https://repository.ugm.ac.id/131557/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72053

Congestive heart failure (CHF) is mainly resulted from heart fibrosis. Collagen accumulation in heart fibrosis occurs as result from activity of myofibroblast. High salt intake activates myofibroblast via angiotensin II - TGF β1 pathway. Telmisartan is a highly selective angiotensin II type 1 (AT1) receptor blocker (ARB) for treatment of hypertension and as partial agonist of PPAR-γ which decreases TGF-β1 and is predicted to prevent myocardial fibrosis. Objective To know the effect of telmisartan in variation doses related TGF-β1 and collagen volume fraction of myocardial tissue. Method Ten-week-old male Wistar Rat (n = 30) were randomized into five groups, and each group consisted of 6 rats. Group 1 : negative control. Group 2 : rats were induced by intake Nacl 8% doses 2% body weight for eight weeks. Group 3 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 3 mg/kgBB for eight weeks. Group 4 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 6 mg/kgBB for eight weeks. Group 5 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 12 mg/kgBB for eight weeks. Myocardial tissue were stained with picrosirius red and collagen volume fraction were assessed by ImageJ programme. Concentration of TGF-β1 in myocardial tissue were assessed by ELISA. ANOVA test followed pos hoc test (Bonferroni test) was used to analyzed each variable. Results Concentration of TGF-β1 in group 1 (909.05 ± 14.28 pg/mL), group 2 (1360.41 ± 56.19 pg/mL), group 3 (1142.75 ± 26.50 pg/mL), group 4 (691.96 ± 34.51 pg/mL) and group 5 (435.97 ± 29.25 pg/mL). TGF- β1 significantly decreased in group 3, 4 and 5 compared in group 2, p = 0.00. Collagen volume fraction in group 1 (2.53 ± 0.11%), group 2( 19.20 ± 0.13%), group 3 (9.35 ± 0.25%), group 4 (3.97 ± 0.38%) and group 5 (2.21 ± 0.29%). Collagen volume fraction significantly decreased in group 3, 4 and 5 compared in group 2, p = 0.00. Conclusion Telmisartan decreases concentration of TGF-β1 and collagen volume fraction of myocardial tissue wistar rats induced by high salt intake.