ISOLASI DAN IDENTIFIKASI SENYAWA SITOTOKSIK FRAKSI ETIL ASETAT DAUN SAMBUNG NYAWA (Gynura Procumbens (Lour.) Merr).
Breast cancer is one of kind malignant disease in Indonesia and the world. The incidence and mortality is still high due to the resistance of the cancer cell, driving the efforts for discovering cancer therapeutic agent that more effectively and safely. The Gynura procumbens pl...
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| Main Authors: | , |
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 出版: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| 主題: | |
| 在線閱讀: | https://repository.ugm.ac.id/128403/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=68746 |
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| 機構: | Universitas Gadjah Mada |
| 總結: | Breast cancer is one of kind malignant disease in Indonesia and the
world. The incidence and mortality is still high due to the resistance of the
cancer cell, driving the efforts for discovering cancer therapeutic agent that
more effectively and safely. The Gynura procumbens plants have activity as
an antipyretic, anti hypotension, anti hypoglycemic and anticancer . The aim
of this research was to find out the cytotoxic potency in ethyl acetate fraction
of Gynura procumbens leaf to MCF - 7 and T47D breast cancer cells and to
identify the active compounds that lead to cytotoxic activity. Gynura
procumbens was extracted using ethanol 96%, then followed by liquid-liquid
partition hexane and ethyl acetate. Ethyl acetate fraction was separated by
two- dimensional TLC method to see existing patches . This method uses
cellulose stationary phase with two different mobile phase is ethanol - water
(1:4) and butanol - acetic acid - water ( 4:1:5 ). Furthermore, ethyl acetate
fraction was separated and propagated by using preparative TLC method, the
results obtained KLTP 3 subfraksi then tested the subfraksi cytotoxic activity
against breast cancer cells MCF-7 and T47D by using MTT assay. The our
results showed that subfraksi 1 have cytotoxic activity against MCF-7 cells
with IC50
value of 605.20 µg / ml. Subfraksi 1 then separated and reproduced
by preparative TLC with a mobile phase of BAW thus obtained isolates and
their structures elucidated. Unpurified isolates obtained so that the
compounds responsible for the cytotoxic activity of G. procumbens is
unknown. Isolates were estimated to contain active compounds similar to 2-
isopropyl-5-methylcyclohexanol (menthol), 2-Cyclohexyl-5-methyl acetate
(acetyl menthol) and 2 - ethylhexyl Phtalate. |
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