IDENTIFIKASI GOLONGAN SENYAWA TOKSIK DARI EKSTRAK METANOLIK DAUN Piper spp.TERHADAP SEL KANKER PAYUDARA T47D
Several species of Piper spp. has know for cytotoxic activities. The activity come from compounds that belong to secondary metabolite. Cis-yagonin and flavokavain B compounds isolated from P. methysticum are having cytotoxic activities to leukemia and ovary cancer cell. The ethanolic extract of P. c...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
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| Online Access: | https://repository.ugm.ac.id/123585/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=63698 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Several species of Piper spp. has know for cytotoxic activities. The
activity come from compounds that belong to secondary metabolite. Cis-yagonin
and flavokavain B compounds isolated from P. methysticum are having cytotoxic
activities to leukemia and ovary cancer cell. The ethanolic extract of P. crocatum
has cytotoxic activity to breast cancer T47D cell line. The metabolites content
from ethanolic extract P. crocatum are alkaloids, saponin, tannin, and flavonoids.
The purposes of the research to analyses cytotoxic activity of Piper spp. and the
compound class which has cytotoxic property are identified. Soxhletation method
with methanol as a solvent was selected for the extraction procedure. The leaves
from 10 species of Piper spp. were collected from Bogor Botanical Garden. MTT
assay was used for cytotoxic assay to cell line T47D. The component from the
extract which had a highest cytotoxicity, was separated by vacuum liquid
chromatography (VLC). The fractions from the VLC with highest cytotoxic
activity was separated by preparative thin layer chromatography (PTLC). TLC
was selected to monitor the constituent from the whole extract, fraction from VLC
and PTLC. The compounds class from part of PTLC with highest cytotoxic
activity are determinated by spray reagents. The highest cytotoxic activity came
from P. porphyrophyllum extract (IC50= 667,5 μg/ml). Cytotoxic activity from the
constituent of the whole extract P. porphyrophyllum less active than the whole
extract and doxorubicin (IC50 VLC fraction= 1.389,14 μg/ml |
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