Anti-hepatitis C virus compounds obtained from Glycyrrhiza uralensis and other Glycyrrhiza species

• Main Authors: Adianti, Myrna, Aoki, Chie, Komoto, Mari, Deng, Lin, Shoji, Ikuo, Sri Wahyuni, Tutik, Inge L, Maria, Soetjipto, Soetjipto, Fuchino, Hiroyuki, awahara, Nobuo, Hotta, Hak
• 格式: Article PeerReviewed
• 語言: English; English; English
• 出版: Wiley-Blackwell 2014
• 主題: RS Pharmacy and materia medica; RS1-441 Pharmacy and materia medica; RS200-201 Pharmaceutical dosage forms
• 在線閱讀: http://repository.unair.ac.id/84661/1/C-003%20Microbiology%20and%20Immunology.pdf; http://repository.unair.ac.id/84661/2/Lembar%20C03.pdf; http://repository.unair.ac.id/84661/3/C03%20-%20Anti-hepatitis%20C%20virus%20compounds%20obtained%20from%20Glycyrrhiza%20uralensis%20and%20other%20Glycyrrhiza%20species.pdf; http://repository.unair.ac.id/84661/; http://onlinelibrary.wiley.com/doi/10.1111/1348-0421.12127/epdf

Development of complementary and/or alternative drugs for treatment of hepatitis C virus (HCV)infection is still much needed from clinical and economic points of view. Antiviral substancesobtained from medicinal plants are potentially good targets to study.Glycyrrhiza uralensisandG. glabrahave been commonly used in both traditional and modern medicine. In this study, extractsofG. uralensisroots and their components were examined for anti-HCV activity using an HCV cellculture system. It was found that a methanol extract ofG. uralensisroots and its chloroform fractionpossess anti-HCV activity with 50%-inhibitory concentrations (IC50) of 20.0 and 8.0mg/mL,respectively. Through bioactivity-guided purification and structural analysis, glycycoumarin,glycyrin, glycyrol and liquiritigenin were isolated and identified as anti-HCV compounds, theirIC50being 8.8, 7.2, 4.6 and 16.4mg/mL, respectively. However, glycyrrhizin, the major constituent ofG. uralensis, and its monoammonium salt, showed only marginal anti-HCV activity. It was also foundthat licochalcone A and glabridin, known to be exclusive constituents ofG. inflataandG. glabra,respectively, did have anti-HCV activity, their IC50being 2.5 and 6.2mg/mL, respectively. Anotherchalcone, isoliquiritigenin, also showed anti-HCV activity, with an IC50of 3.7mg/mL. Time-of-addition analysis revealed that allGlycyrrhiza-derived anti-HCV compounds tested in this study act atthe post-entry step. In conclusion, the present results suggest that glycycoumarin, glycyrin, glycyroland liquiritigenin isolated fromG. uralensis,as well as isoliquiritigenin, licochalcone A and glabridin,would be good candidates for seed compounds to develop antivirals against HCV.