การสังเคราะห์อนุภาคนาโนซีลีเนียมที่เสถียรด้วยไคโตซานและไลโครีนเพื่อนำส่งยา

• Main Author: ปัณฑารีย์ ปิ่นประสงค์
• Other Authors: นงนุช เหมืองสิน
• Format: Senior Project
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2015
• Subjects: ซีลีเนียม; อนุภาคนาโน; ไคโตแซน; ระบบนำส่งยา; เภสัชกรรมเทคโนโลยี
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:9913
• Institution: Chulalongkorn University

Selenium nanoparticles attracted more attention due to anticancer activities; however, the nanoparticles are naturally unstable. Therefore, in this work, selenium nanoparticles were prepared using modified chitosan as stabilizer to enhance their stability. Chitosan was modified with lycorine via the tosylation reaction to obtain tosyl-lycorinechitosan (Ts-Ly-chitosan). Tosyl groups of Ts-Ly-chitosan were removed by substitution with 5-amino-2-mercaptobenzimidazole (AMB) to obtain AMB-Ly-chitosan. Both modified chitosans were characterized by ¹H-NMR spectroscopy, FT-IR spectroscopy and thermogravimetric analysis. Furthermore, both modified chitosans were used as stabilizer of selenium nanoparticles which were characterized by scanning electron microscope (SEM) and showed spherical shape. In addition, the size and zeta potential of selenium nanoparticles stabilized by Ts-Ly-chitosan were determined by dynamic light scattering (DLS) to be about 80 nm and +40 mV, respectively. The cytotoxicity of selenium nanoparticles were evaluated against cancer cells by MTT assay. The results showed that the cytotoxic against KB, oral cancer cells of selenium nanoparticles stabilized by Ts-Ly-chitosan (IC₅₀ = 3.907 µM) were higher than selenium nanoparticles stabilized by AMB-Ly-chitosan.