ศึกษาอันตรกิริยาระหว่างเยื่อไขมันสองชั้นกับอนุภาคออกซิไดส์คาร์บอนระดับนาโน

• Main Authors: กมลลักษณ์ สาธรสันติกุล, ฐิติพร แสนสุรีย์รังสิกุล
• Other Authors: ศุภศร วนิชเวชารุ่งเรือง
• Format: Senior Project
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2014
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:9856
• Institution: Chulalongkorn University

Drug carriers have received much interest and have been applied for therapeutic, scientific studies and food/beverage industries. Herein, Oxidized Carbon Nanoparticles (OCN) are synthesized. The particles can deliver macromolecules into the cells. In this work, we have studied the mechanism of lipid bilayer membrane penetration of OCN. OCN was synthesized by strong oxidation of graphite under sonication. The particles were separated by step-wise high-speed centrifugation. The OCN showed a spherical shape with a diameter of 37.7± 4.6 nanometers. We have mimicked lipid bilayer membrane of a cell through the preparation of cell-size liposomes. The liposomes contained donor lipid fluorescence dye at their lipid membrane. We loaded the acceptor lipid fluorescence dye onto OCN particles. These were used for studying the interaction between lipid bilayer membrane and OCN through the Förster resonance energy transfer (FRET) technique. By monitoring the fluorescence transformation, we observed that OCN possessed good affinity to the lipid bilayer membrane. To confirm that the particles could create pores on the lipid bilayer membrane of the liposomes and thus induced leakage of the liposomes, we fabricated liposome suspension in which the content of the solution at the inside and that at the outside of the liposome were different. Then we investigated, under fluorescence microscope, an ability of OCN to destroy such difference. It was observed that after 60 minutes of introducing OCN into the liposome suspension, the content of the solution at the inside of the liposomes became similar to that at the outside of the liposomes. The result confirms the hypothesis that the OCN can induce transient pores onto the lipid bilayer membrane.