การศึกษาเซอรูโลพลาสมินเพื่อเป็นตัวบ่งชี้ชีวภาพของปริมาณตะกั่วในเลือด

• Main Author: วารินทร์ ลีลาคุณากร
• Other Authors: สุกัญญา สุนทรส
• Format: Theses and Dissertations
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 1998
• Subjects: ตะกั่วในร่างกาย; ตะกั่วเป็นพิษ; เซอรูโลพลาสมิน; Lead in the body; Lead poisoning; Ceruloplasmin
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:58782
• Institution: Chulalongkorn University

Blood lead level alone can not be used to diagnose lead toxicity precisely in human, due to different resistance in each individual and a short half life of lead in blood circulation (28 - 36 days). Enzyme aminolevulinic acid dehydratase (ALA-D) has been routinely used for screening of lead intoxication. However, variation of the activity with age limits the use of ALA-D. Therefore, enzyme(s) serve as biomarker for lead toxicity would be advantage. Ceruloplasmin is a copper-transporting protein in blood which 6-8 atoms of Cu bind to and serve as important co-factor for oxidase activity (Kaldor, 1983). The copper can be replaced by lead with a concomitant decrease in oxidase activity (Rosawan, 1996). Therefore, this protein could be served as lead intoxication biomarker. This study shows the relationship between the blood lead level and the activities of ALA-D and ceruloplasmin’s oxidase from workers from battery and printing factories compared to control group. Both enzyme activities are reduced when blood lead level is increased (threshold of 10.0 and 12.5 ug/100 ml respectively), with correlation coefficient of -0.8643 and -0.6785 which accurately estimates the blood lead level of 74.7% and 46.0%, respectively. When lead level are less than 20 ug/100 ml, correlation coefficient of -0.5785 and -0.6963 suggesting that ceruloplasmin may serve as a better biomarker than ALA-D. Moreover, ceruloplasmin is more stable at -20 ° c, more than 2 weeks, compared to less than 7 days in latter. The procedure for ceruloplasmin activity determination is also much more convenient and rapid.