ผลของสารสกัดจากเชื้อรา Glomerella cingulata ต่อการเจริญของเปล้าน้อยที่ได้จากการเพาะเลี้ยงเนื้อเยื่อ

• المؤلف الرئيسي: ศศิ มะลิพันธุ์
• مؤلفون آخرون: นลิน นิลอุบล
• التنسيق: Theses and Dissertations
• اللغة: Thai
• منشور في: จุฬาลงกรณ์มหาวิทยาลัย 2000
• الموضوعات: เปล้าน้อย (พืช) > โรค
• الوصول للمادة أونلاين: https://digiverse.chula.ac.th/Info/item/dc:35989
• المؤسسة: Chulalongkorn University

Glomerella cingulata causing leaf spot on Croton sublyratus Kurz. gave maximum sporulation on day 7 when grown on Czapek s Agar. The spores were used as the source of toxin production quantitative comparison of toxin production by Glomerella cingulata grown in two different media showed that in Czapek s Dox medium containing 0.1% yeast extract, indoleacetic acid (IAA) and phenyl acetic acid (PAA) at 0.004 and 1.58 mg/l of culture broth were produced, respectively, which were higher than those in Richard s medium in which only 0.003 and 0.046 mg/l of IAA and PAA were produced, respectively. Toxicity test of the commercial available IAA and PAA showed positive effect on callus of Croton sublyratus Kurz. Both compounds had additive effects. Induction for resistance of Croton sublyratus Kurz. to the toxin extract from Glomerella cingulata was performed by challenging the callus and shoot to the increasing concentrations of the crude extract from this fungus. Callus and shoot resistant to higher concentration of the extract from 1500 ppm containing IAA at 6.645 mg/l and PAA at 75.75 mg/l to 2000 ppm containing IAA at 8.86 mg/l and PAA at 101.00 mg/l were obtained. At this concentration, about 91% and 92% of callus and shoot were killed, respectively.