ความหลากหลายทางพันธุกรรมของเห็ดโคน Termitomyces sp. บนพื้นฐานของการวิเคราะห์ลำดับพันธุกรรมบริเวณไอทีเอส
Termite mushroom (Termetomyces sp) were surveyed and collected from 18 areas in Kanchanaburi and Burirum province where large populations of termite mushroom were found. Morphological studies confined samples into 5 subgroups: Termitomyces clypeatus R. Heim Termitomyces aurantiacus Termitomyces ento...
Saved in:
| 主要作者: | |
|---|---|
| 其他作者: | |
| 格式: | Theses and Dissertations |
| 語言: | Thai |
| 出版: |
จุฬาลงกรณ์มหาวิทยาลัย
2004
|
| 主題: | |
| 在線閱讀: | https://digiverse.chula.ac.th/Info/item/dc:35630 |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|
| 機構: | Chulalongkorn University |
| 語言: | Thai |
| 總結: | Termite mushroom (Termetomyces sp) were surveyed and collected from 18 areas in Kanchanaburi and Burirum province where large populations of termite mushroom were found. Morphological studies confined samples into 5 subgroups: Termitomyces clypeatus R. Heim Termitomyces aurantiacus Termitomyces entolomoides Termitomyces striatus and Termitomyces globules. Further studies on nucleotide sequence was carried out. DNA were extracted from gills using CTAB method and DNA yields with their OD260/OD280 ratio varied in the range of 1.7 to 1.9. The amplification of DNA using ITS4 and ITS5 specific to ITS regions of SSU rDNA resulted in amplified products of approximately 638 nucleotides to 17 out of 18 samples from Kanchanaburi and 948 nucleotides in 1 sample from Burirum. DNA fragments were then cloned into pCRII plasmid using TA cloning strategy. And colonies resistant to antibiotic were selected from at least 60 colonies per samples. The cloned DNAs were investigated for their accurate inserted fragments via Genome Direct to View Kit, small scale plasmid preparation and PCR amplification using ITS4 and ITS5 primers. Three clones each per samples were then analyzed for their nucleotide sequence using dye termination method. It was found that DNA sequence from the first and second group are 623-646 nucleotides and 948 nucleotides respectively. DNA sequence comparison via sequence alignment revealed a distinguish sequence characteristic when compared with the foreign termite mushroom but similar among the samples. Phylogenetic relationships when analyzing using PAUP revealed 5 subgroup discrimination which distantly separated from foreign samples. The relationships of T. entolomoides and T. clypeatus were arranged in correlation with the identification using morphological study where as the others were not, however it was found that termite mushroom from Burirum were genetically in distance from those of Kanchanaburi |
|---|