การสกัดเปลาโนทอลด้วยคาร์บอนไดออกไซด์ภาวะเหนือวิกฤต

• Main Author: อุษณีย์ ดวงหฤทัยกุล
• Other Authors: อมร เพชรสม
• Format: Theses and Dissertations
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2006
• Subjects: เปล้าน้อย (พืช) > การวิเคราะห์; การสกัดด้วยของไหลวิกฤตยิ่งยวด; คาร์บอนไดออกไซด์; เปลาโนทอล
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:35306
• Institution: Chulalongkorn University

Extraction of plaunotol from plau-noi leaves was carried out using supercritical carbon dioxide (SC-CO[subscript 2]). The effect of process parameters on extraction efficiency were investigated, namely pressure (255, 265, 275 bar), temperature (40, 50, 60 degree celsius), carbon dioxide flow rate (3, 5 g/min), co-solvent, and particle size of raw material. The optimum process condition of supercritical carbon dioxide for plaunotol was determined. The results indicated that % plaunotol was significantly increased with an increase in pressure, temperature and % plaunotol content was significantly increased with an increase in carbon dioxide flow rate. The optimum condition of extraction was 275 bar, 50 degree celsius, 3 g/min without co-solvent, The plaunotol content were 0.24 % (w/w) on basic of dry leaves and 6.7 % (w/w) in crude extract, respectively. The particle size of raw material did not affect on extraction. These results were compared with those of classical solvent extraction. It was found that SC-CO[subscript 2] extraction of plaunotol and its content might carried out at higher yield, shorter time, using nontomic, less solvent and less cost than those classical solvent extraction. Purification of plaunotol with quick column chromatography increased plaunotol content to 53 % (w/w) and purification of plaunotol with column chromatography further increased plaunotol content to 69.2 % (w/w).