การผลิตชุดตรวจคัดกรองโรคพราเดอร์-วิลลี่โดยอนุภาคทองคำนาโนเมตร
This study aims to generate and optimize DNA lateral flow strip test for Prader-Willi syndrome (PWS) screening. The test strip interpretation is based on the unique optical properties of gold nanoparticles (AuNP) and the efficiency of chromatographic separation. This system enables visual detection...
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| Format: | Theses and Dissertations |
| Language: | Thai |
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จุฬาลงกรณ์มหาวิทยาลัย
2011
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| Online Access: | https://digiverse.chula.ac.th/Info/item/dc:29860 |
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| Institution: | Chulalongkorn University |
| Language: | Thai |
| Summary: | This study aims to generate and optimize DNA lateral flow strip test for Prader-Willi syndrome (PWS) screening. The test strip interpretation is based on the unique optical properties of gold nanoparticles (AuNP) and the efficiency of chromatographic separation. This system enables visual detection of results without specialized equipment. AuNPs were synthesized by citrate reduction method. The AuNPs was characterized with UV-vis spectroscopy and transmission electron microscope. A thiolated oligonucleotide was used for conjugation on AuNPs. The lateral flow strip test was prepared, assembled and optimized. The results showed that detection limit of 0.6 fmol synthetic target DNA methylation and 8 fmol synthetic target DNA unmethylation were obtained under optimal experimental conditions. Lateral flow strip test could detect Methylation/Unmethylation synthetic target and DNA methylation from PWS DNA lateral flow strip test for PWS diagnosis could be used to discriminate between unmethylation and methylation from DNA target and MSP product. This study provides a new model for detection of epigenetic change and could be applied in further translation research. |
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