ผลของอนุภาคทองคำระดับนาโนเมตรต่อการสร้างหลอดเลือดฝอย โดยมุ่งเน้นศึกษาความเปลี่ยนแปลงที่เซลล์เพอริไซต์

• Main Author: ศศิกานต์ ลู่ประเสริฐกุล
• Other Authors: เดภิชา จินดาทิพย์
• Format: Theses and Dissertations
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2019
• Subjects: หลอดเลือดแดง; ทอง; การเปลี่ยนสภาพของเซลล์; ArteriesCell differentiation; Gold; Cell differentiation
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:29162
• Institution: Chulalongkorn University

Pericytes are perivascular cells that wrap around endothelial cells to form wall of capillaries and post-capillary venules. These cells play important roles in physiological and pathological processes, including angiogenesis. Nowadays, gold nanoparticles (AuNPs) are used for anti-angiogenesis by inhibiting the activity of endothelial cell proliferation, migration, and tube formation. Nevertheless, the effects of gold nanoparticles on the alteration of pericyte morphology and their functions have not been well explored. In this study, 20 nm-AuNPs were applied to human placenta pericyte monoculture with concentration of 30 ppm. Morphology of pericytes was observed by transmission electron microscopy. Cell migration was evaluated by transwell migration assay. Real-time RT-qPCR was used to assess Ki-67 and PDGFR-β mRNA expression. Additionally, these AuNPs 30 ppm-treated pericytes were co-cultured with human umbilical cord endothelial cells on Matrigel to explore the 3D tube formation. The results showed that AuNPs significantly suppressed migration, proliferation, and PDGFR-β mRNA expression of pericytes. The ultrastructure of pericytes displayed AuNPs in several organelles, i.e., engulfed late endosomes, lysosomes, and mitochondria. Interestingly, these mitochondria were swollen or damaged. Furthermore, the number of capillary tube formations was essentially reduced. A number of pericytes on these capillary tubes were round shape and did not extend the processes along the tube, which in turn increased incomplete tube formation compared with the control. In summary, AuNPs could affect pericyte biology, including proliferation, migration, morphology, and tube formation property. These findings may help support the potential application of AuNPs in anti-capillary tube formation by pericyte-targeted therapy.