การสกัดแยกเฮปารินจากเนื้อเยื่อสัตว์ : รายงานผลการวิจัยฉบับสมบูรณ์

• Main Author: ศุภศร วนิชเวชารุ่งเรือง
• Format: Research Report
• Language: Thai
• Published: สถาบันเทคโนโลยีชีวภาพและวิศวกรรมพันธุศาสตร์ จุฬาลงกรณ์์มหาวิทยาลัย 1999
• Subjects: เฮปาริน; เซลล์และเนื้อเยื่อสัตว์
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:15444
• Institution: Chulalongkorn University

Study show that the most suitable tissue source for heparin extraction is porecine lung. The extraction and purification processed of heparin from porcine lung consist of 1) tissue autolysis 2) tissue disgestion by Neutrase enzyme 3) precipitation of impurities with trichloroacetic acid 4) removal of molecules weight of less than 3,000 daltons by ultrafiltration 5) fractional precipitation of contaminating glycosaminoglycans and heparin with aqueous ethanol solution and 6) further purification of the obtained heparin by anion exchange chromatography. It was found that the Anti Factor Xa blood anticoagulant activity of the purified heparin was 143.21 unit/mg. By using these extraction and purification processes, one kilogram (wet weight) of the porcing lung tissue yields 18.20 milligrams of high purify heparin (2,606.42 units). Structural analyses of the obtained heparin were done by nuclear magnetic resonance spectroscopy and elemental analysis. It was found that heparin preparation had 4.63%N 48.86%C 9.31%H and 37.20%S and molecular weight of 4,500 daltons estimated by size exclusion chromatography.