อันตรกิริยาของพืชสมุนไพรที่มีฤทธิ์ต้านเบาหวานในพื้นที่ของโครงการอนุรักษ์พันธุกรรมพืช อันเนื่องจากพระราชดำริฯ กับหน้าที่การทำงานของพี-ไกลโคโปรตีน : รายงานวิจัย

• Main Authors: สุรีย์ เจียรณ์มงคล, นนทิมา วรรธนะภูติ
• Format: Research Report
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2015
• Subjects: สมุนไพร; สารสกัดจากพืช
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:13585
• Institution: Chulalongkorn University

The purpose of this study was to investigate and compare the effects of 4 herbal plants in the Plant Genetic Conservation Project area under The Royal Initiative of Her Royal Highness Princess Maha Chakri Sirindhorn on the drug absorption through intestinal restrictive barrier, using the in vitro model of the Caco-2 cells. The plants were selected into this study by its ability to inhibit α-glucosidase, which is a drug target for diabetic control. These plants included Pterospermum littorale Craib (Family Sterculiaceae); Dialium cochinchinense Pierre (Family Fabaceae); Mamecylon plebejum Kurz. var. ellipsoideum Craib. (Family Melastomataceae) and Thespesia populnea (L.) Soland.ex Corr. (Family Malvaceae). The effects of plant extracts on paracellular transport and their interaction with P-glycoprotein (P-gp) were determined. The restrictive integrity of Caco2 monolayers was assessed by transepithelial electrical resistant (TEER) values and Lucifer yellow transport. The results demonstrated that all of the plant extracts at the highest concentration in this study had no disruptive effect on the integrity and restrictive barrier property of the Caco-2 monolayers. The findings suggested that these extracts would not affect the tight junction integrity. Thus, they would be unable to facilitate the paracellular transport pathway. The interference on the absorption of P-gp substrate will be studied further.