การศึกษาปัจจัยที่เหมาะสมในการสังเคราะห์อนุภาคทองนาโนด้วยวิธีการทางชีวภาพ

• Main Author: วิธิดา จันยะมิตรี
• Other Authors: สริสา ณ ป้อมเพ็ชร์
• Format: Senior Project
• Language: Thai
• Published: จุฬาลงกรณ์มหาวิทยาลัย 2020
• Subjects: อนุภาคนาโน > การสังเคราะห์; ทอง; Nanoparticles > Synthesis; Gold
• Online Access: https://digiverse.chula.ac.th/Info/item/dc:10783
• Institution: Chulalongkorn University

Biosynthesis of gold nanoparticles (AuNPs) is environmental friendly, non-toxic, and costeffective. In the biosynthetic process, parameters of the physicochemical reaction greatly influenced with respect to shape and size. Thus, in this study, we aimed to biosynthesize AuNPs using Aspergillus niger MSCU 0361 and optimize{u1001F0}the parameters related to the biosynthesis. This fungus was able to synthesize AuNPs when challenged with tetra-chloroauric acid (HAuCl₄). The optimal condition for AuNP synthesis was 30 g of fungal biomass, 0.02 M of HAuCl₄ in acetate buffer solution pH 4.70, and room temperature incubation. The UV-vis spectra of AuNPs showed unique optical characteristics at 530-570 nm, and the transmission electron microscopy (TEM) images revealed that the AuNPs were found to be polydispersed with different shapes such as spherical, triangular, polygonal, and uncertain shapes. The hydrodynamic diameter and the size distribution of AuNPs were observed by dynamic light scattering (DLS) technique. The average size measured by DLS was 35.37 nm. In addition, DLS analysis was able to determine the distribution of AuNPs reported as polydispersity index (PDI), which showed the PDI value of 0.655. The zeta potential measurement of AuNPs was -17.5 mV. In summary, this research demonstrated that A. niger MSCU 0361 could synthesize AuNPs through optimization and modification of the physicochemical parameters of the reaction. However, the mechanism for the biosynthesis of AuNPs is unclear, and it may also involve with other relevant factors. Therefore, further studies are needed to obtain optimal biosynthetic conditions.