Soluble expression of human leukemia inhibitory factor with protein disulfide isomerase in Escherichia coli and its simple purification

• Main Authors: Jung A.S., Koo B.-K., Chong S.-H., Kim K., Choi D.K., Vu T.T.T., Nguyen M.T., Jeong B., Ryu H.-B., Kim I., Jang Y.J., Robinson R.C., Choe H.
• Other Authors: BIOCHEMISTRY
• Format: Article
• Published: 2019
• Subjects: endotoxin; glycine; hybrid protein; leukemia inhibitory factor; protein disulfide isomerase; proteinase; TEV protease; unclassified drug; amino terminal sequence; animal cell; article; controlled study; disulfide bond; embryonic stem cell; Escherichia coli; low temperature; mass spectrometry; mouse; nonhuman; pluripotent stem cell; protein analysis; protein cleavage; protein domain; protein expression; protein modification; protein purification; solubility; Amino Acid Sequence; Gene Expression; Gene Order; Humans; Leukemia Inhibitory Factor; Mass Spectrometry; Molecular Sequence Data; Plasmids; Protein Disulfide-Isomerases; Protein Stability; Recombinant Fusion Proteins; Solubility
• Online Access: https://scholarbank.nus.edu.sg/handle/10635/161443

10.1371/journal.pone.0083781