Sistem Analisis Suntik Alir Biamperometri untuk Penetapan Kadar Vitamin: A bipotentiometric fia system for determination of vitamin C

• 主要作者: Perpustakaan UGM, i-lib
• 格式: Article NonPeerReviewed
• 出版: [Yogyakarta] : Universitas Gadjah Mada 2003
• 主題: Jurnal i-lib UGM
• 在線閱讀: https://repository.ugm.ac.id/24663/; http://i-lib.ugm.ac.id/jurnal/download.php?dataId=7641

A bipotentiometric FIA system has been developed for the determination of vitamin C. The system was a single stream manifold, which consisted of a peristaltic pump, a self made injector with internal loop and two Pt-electrodes connected to a PC by serial ADC or parallel ADC interface card. One of the Pt-electrodes was located in the injector before the internal loop, while the other electrodes at the end of the TEFLON reaction tube. The PC was supported with a self-made software for controlling the pump, signal acquisition, processing, and visualisation, and further peak determination, followed by calibration of standard solutions and calculation of sample concentrations. The above HA-system configuration was applied to determine vitamin C by the injection of sample solution into a stream of potassium hexacyanoferrate (III) solution. The optimum result was achieved with knitted tube reaction of 0.8 mm i.d., 50 cm length, at flow rate 0,64 ml/minute, and consumed 3.6 ml of carrier solution per five injection, and gave linearity range at 0.001 0.100 N vitamin C, with the coefficient of correlation 0.9999, RSD peak to peak better than 3.0% and the error of artificial samples were less than 3.4 %. Key Words : Bipotentiometric-FIA, injector with internal loop, module-program, vitamin C ABSTRAK Telah dikembangkan suatu sistem analisis suntik alir bipotensiometri untuk penetapan kadar vitamin C. Sistem mempunyai manifold jalur tunggal dan terdiri dari pompa peristaltik, injector dengan loop internal buatuan sendiri dan dua elektrode Pt yang dihubungkan ke PC melalui ADC gawai antar muka secara serial atau paralel. Salah satu elektrode Pt ditempatkan di dalam injector, sebelum loop internal, dan elektrode Pt yang lain ditempatkan pada ujung akbir jalur reaktor, yang terbuat dan TEFLON. PC dilengkapi dengan perangkat lunak buatan sendiri, untuk mengendalikan pompa, untuk akuisasi, mengolah dan menggambarkan sinyal, dan selanjutnya untuk kalibrasi larutan balm dan menghitung kadar sampel. Konfigurasi sistem analisis suntik alir ini diterapk an untuk penetapan kadar vitamin C, dengan menyuntikkan larutan sampel ke dalam aliran pereaksi heksasianoferat(III). Hasil optimum didapat dengan jalur reaksi, yang dirajut