Biosynthesis of A6'7-anhydroerythromycin via enoyl reductase inhibition by isonicotinic hydrazide (INH)
Isonicotinic hydrazide (INH) was added to a fermentation of mutant of Saccharopolyspora erythraea ATCC 11912 to inhibit the activity of 2,4,6,8-tetramethyI-7,9-dihydroxy-2-en-5-on-undecyl-ACP enoyl reductase, an enzyme which catalyses enoyl reduction process of an intermediate in the erythronolide b...
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| Format: | Article NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
1999
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| Online Access: | https://repository.ugm.ac.id/17856/ http://i-lib.ugm.ac.id/jurnal/download.php?dataId=624 |
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| Institution: | Universitas Gadjah Mada |
| Summary: | Isonicotinic hydrazide (INH) was added to a fermentation of mutant of Saccharopolyspora erythraea ATCC 11912 to inhibit the activity of 2,4,6,8-tetramethyI-7,9-dihydroxy-2-en-5-on-undecyl-ACP enoyl reductase, an enzyme which catalyses enoyl reduction process of an intermediate in the erythronolide biosynthesis, and produce A"3-anhydroerythromycin derivative. The. optimum INH additional concentration of above 0.1% was able to inhibit the enzyme activity, and produce A6�7-anhydroerythromycin derivative as shown by FT-infrared spectrophotometric analysis of the product after its purification using thin layer chromatography.
Keywords: Isonicotinic hydrazide (INH) enoyl reductase � d7-anhydroerythromycin � mutant Saccharopolyspora |
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