PRODUCTION AND APPLICATION OF KERATINASE ENZYME FROM 4 STRAINS OF BACILLUS SPP. ISOLATED FROM YOGYAKARTA AND GARUT CITY
Recently, chicken feathers have been discarded in bulk as waste from poultry processing industries. Utilization of chicken feathers as sources of protein for animal feed challenge us as an effort to minimize the environmental impact of this waste for creating environmentally friendly farming indus...
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| Main Authors: | , , , |
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| 格式: | Article PeerReviewed |
| 語言: | English |
| 出版: |
2016
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| 主題: | |
| 在線閱讀: | https://repository.ugm.ac.id/139208/1/Production%20and%20Application%20of%20Keratinase%20Enzyme%20from%204%20Strains%20of%20Bacillus%20spp%20isolated%20from%20Yogyakarta%20and%20Garut%20City.pdf https://repository.ugm.ac.id/139208/ |
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| 總結: | Recently, chicken feathers have been discarded in bulk as waste from poultry processing
industries. Utilization of chicken feathers as sources of protein for animal feed challenge us as an effort to
minimize the environmental impact of this waste for creating environmentally friendly farming industry.
Chicken feather waste treatment involves the role of the enzyme keratinase through the process of
hydrolysis because the chicken feathers containing keratin. Keratinase enzyme can be produced from
several microorganisms such as fungi and bacteria. Therefore, enzyme keratinase expected to be produced
from Bacillus spp. which has been previously isolated from Yogyakarta and Garut City. The purpose of this
research was to determine the production of keratinase enzyme produced from Bacillus spp. and apply the
keratinase enzyme in the process of degradation of chicken feathers. Research is consisting of cultivation
of Bacillus spp., measuring the growth of Bacillus spp., investigation of degradation of chicken feathers by
Bacillus spp., and the production of keratinase enzyme produced by Bacillus spp. Keratinase enzyme
production data at varying feather analyzed using completely randomized factorial design 4x2x4
(Completely Randomized Design), and then if there are differences continued with test Duncans New Multiple
Range Test (DMRT). This analysis compares the keratinase enzyme produced by each isolate with different
types of bacterial isolates are used, the addition of the substrate in the medium, and the difference in
incubation time of each isolates of Bacillus spp. From the results obtained bacterial growth and the ability
of degradation which is best performed by Bacillus sp. TD5B. The best enzyme production was obtained on
medium supplemented with chicken feathers as substrate. |
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