KARAKTER FENOTIP DAN MOLEKULAR MELON (Cucumis melo L. �TACAPA�) PADA MEDIA TANAM TANAH KARST
Yogyakarta district has 158.600 ha critical land and spread off in three Agro Ecosystem zones. Two of them are karsts critical land. Critical lands which contain calcium carbonate in high concentration and water dehydration in upper surface give abiotic stress in wide range of plant. Melon cultivar...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/134316/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=75401 |
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| Summary: | Yogyakarta district has 158.600 ha critical land and spread off in three Agro
Ecosystem zones. Two of them are karsts critical land. Critical lands which
contain calcium carbonate in high concentration and water dehydration in upper
surface give abiotic stress in wide range of plant. Melon cultivar TACAPA has
superior characteristic derived from parental crossing, � Action 434 and � PI
371795 and potential to be developed in karsts critical land. Abscicic acid (ABA)
is a phytohormone expressed by plant in abiotic stress condition. CmBG1 is a
gene which regulate ABA hormone in melon. The purposes of this research were
examining the phenotipical and molecular characters of melon cultivar TACAPA
in response to karsts critical land in order to study molecular characterization of
CmBG1 gene. Analysis was done qualitatively by using Reverse Transcriptase-
PCR (RT-PCR) and Electrophoresis, while quantitative analysis was conducted by
observing absorbance score in spectrophotometer. CmBG1 gene expression is
examined by using Real time PCR (qPCR). Result showed that almost
morphological characters of melon such as plant height, amount of leaves, fruit
weight and seed production have no significant differences with melon in control
plantation media. Significantly difference results were showed in flowering time
and water loss degree. Molecular characters obtained are CmBG1 detected in size
±1258 bp, CmBG1 gene concentrations in melon which planted in control media
are lower than melon in critical lands media. These results are similar with the
real time quantitative analysis method. It also be revealed that melon TACAPA is
more potential plant compared to another cultivar that can be developed in karst
critical land area. |
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