ISOLASI DAN IDENTIFIKASI SENYAWA PENANGKAP RADIKAL 2,2-DIFENIL-1-PIKRIHIDRAZIL DARI KULIT BATANG BIDARA (Zizyphus mauritiana Auct. non Lamk.)
The presence of excessive free radicals often leads to many degenerative diseases. This study aimed to isolate and identify the antioxidant compounds from the stem bark of Jujube tree (Zizyphus mauritiana Auct. non Lamk.). Isolation was conducted by bioassay guided fractionation, while antioxidant a...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/133341/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=73955 |
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| Summary: | The presence of excessive free radicals often leads to many degenerative
diseases. This study aimed to isolate and identify the antioxidant compounds from
the stem bark of Jujube tree (Zizyphus mauritiana Auct. non Lamk.).
Isolation was conducted by bioassay guided fractionation, while antioxidant
activity was assayed by DPPH radical scavenging method. The methanol extract
of the stem bark was triturated into fraction n-hexane, ethyl acetate and methanol
extract residue and all of them were examined for their DPPH radical scavenging
antioxidant activity. The ethyl acetate fraction were then separated by repeated
vacuum liquid chromatography (VLC) and purified using preparative TLC to
obtain the active isolate. The purity of the isolate was evaluated by TLC method
and melting point measurement. The structure of the isolate was identified
chemically using various spray reagent and UV-Vis, IR, LC-MS and 1H-NMR
spectroscopic method. The methanol extract, n-hexane fractions, ethyl acetate
fraction, methanol extract residue and fractions derived from the separation of
ethyl acetate fraction were determined for their total phenolic and flavonoid
content.
The results showed that the methanol extract residue containing the highest
total phenolic (36.29 % w/w EGA), while E4 fraction containing the highest total
flavonoids (21.31% w/w EQ). The active isolate having the DPPH radical
scavenging activity with IC50 of 7.58 ï�/mL. Based on spectroscopic analysis,
active isolate contains triterpene compound which condense with p-coumaroyl
group and their have trans configuration. |
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