PENGARUH LAMA PERENDAMAN DALAM AIR TERHADAP SITOTOKSISITAS FIBER REINFORCED COMPOSITE JENIS POLYETHYLENE FIBER PADA SEL FIBROBLAS (METODE MTT)
Fiber reinforced composite (FRC) is a material made of polymer matrix that is reinforced by fibers. Polyethylene fiber is one of the commonly used fiber due its good properties. The use of FRC in the oral cavity promotes a sustainable interaction between the FRC and saliva which is mostly consists o...
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| Main Authors: | , |
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| Format: | Theses and Dissertations NonPeerReviewed |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| Subjects: | |
| Online Access: | https://repository.ugm.ac.id/131774/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72277 |
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| Summary: | Fiber reinforced composite (FRC) is a material made of polymer matrix
that is reinforced by fibers. Polyethylene fiber is one of the commonly used fiber
due its good properties. The use of FRC in the oral cavity promotes a sustainable
interaction between the FRC and saliva which is mostly consists of water.
Polymer matrix absorbs water and facilitate a monomer release which can cause a
cytotoxic effects to human cells. The purpose of this study was to determine the
effect of immersion duration in water to cytotoxicity of polyethylene FRC on
fibroblasts.
Flowable composite (MasterFlow, Biodinamica, Brazil) and polyethylene
fiber (CONSTRUCT Kerr) are material used in this study. Samples which sized
2x2x5 mm (n=8) immersed in 2 ml of water for 0,1,and 7 days. The water extracts
were added in to the culture of fibroblast cells (Vero cell line). Cytotoxicity test
were done by using the MTT method. The optical density was calculated using the
ELISA Plate reader to determine the percentage of surviving cells. Data were
statistically analyzed by One Way ANOVA and LSD0.05.
The results showed an average viability of fibroblasts of the water
immersion group (0,1,7 days) were86.713+2.297%, 77.082+3.388%,
74.079+3.350%. The viability of fibroblasts were decreased from day 0 to day 7.
The result of One Way ANOVA test showed a significant influence between
immersion duration and viability of fibroblasts (p<0.05). LSD0.05showed
significant differences between treatment groups except 1 and 7 days (p>0.05).
The conclusion of this study: immersion duration of Polyethylene FRC has
significant effect against cytotoxicity on fibroblast.
Keyword: polyethylene FRC, water immersion, cytotoxicity, MTT method,
fibroblast |
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