IDENTIFIKASI MOLEKULER NEUTROPHIL GELATINASE ASSOCIATED LIPOCALIN (NGAL) PADA MODEL HEWAN TIKUS (Rattus norvegicus) YANG DIINDUKSI GAGAL GINJAL AKUT DENGAN GLISEROL
Examination standards that are commonly used to monitor the development of acute renal failure (ARF) such as serum levels of creatinine and urea are less sensitive and less specific. Biomarker that can be used for the detection of ARF earlier and accurately is neutrophil gelatinase-associated lipoca...
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| 格式: | Theses and Dissertations NonPeerReviewed |
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[Yogyakarta] : Universitas Gadjah Mada
2014
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| 總結: | Examination standards that are commonly used to monitor the
development of acute renal failure (ARF) such as serum levels of creatinine and
urea are less sensitive and less specific. Biomarker that can be used for the
detection of ARF earlier and accurately is neutrophil gelatinase-associated
lipocalin (NGAL). The objective of this research was to identify NGAL as
biomarker of acute renal failure by comparing the levels of serum ureum and
creatinin in rats induced acute renal failure with 50% glycerol.
Twelve male Wistar rats (Rattus norvegicus), 2 months of age with 150-
200 g body wight (bw), were divided into 2 groups. Group I as the control group
(n=2) were injected with sterile distilled water 10 mL/kg bw intramuscular and
group II (n=10) were injected with 50% glycerol 10mL/ kg bw intramuscular,
(after 12 hours of fasting). At 0, 6, 12, and 24 hours after induction, blood were
collected from retroorbitalis for analysis of urea, creatinine concentration and
identification of NGAL. The rats in group II were necropsied every 2 rats and
removed the kidney of each after time blood collection for histopathologic process
and NGAL identification. The levels of creatinin and ureum were analyzed by
independent t test. Identification of NGAL was conducted by one step reverse
transcriptase (RT)-polymerase chain reaction (PCR) and sequencing. The renal
histopathologic changes were examined with a microscope 400x magnification.
The results showed that glycerol induction could elevated the levels of
urea at 6 hours, 12 hours and 24 hours significantly in compared to the control
group (p<0.05). Creatinine levels were increased significantly at 24 hours in
compared to the control group (p<0.05). The expression of NGAL in kidney and
blood samples could be detected at 6 hours, 12 hours and 24 hours after induction.
Intramuscular injection of 50% glycerol in rats caused tubular cell necrosis,
tubular lumen dilatation, eosinophilic mass in the tubular lumen, and mononuclear
cell infiltration in the interstitial cells. In conclusion NGAL could be detected
before increasing of creatinine levels and could be used as an early detection of
ARF |
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