DETEKSI GEN KETAHANAN TERHADAP Cucumber mosaic virus PADA JAGUNG (Zea mays L.)

• Main Authors: , REFI SEFGIANA, , Dr. Budi Setiadi Daryono, M.Agr.Sc.
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/129924/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=70333

Maize (Zea mays L.) is one of the most important crops, because as source of carbohydrates, besides wheat and starch. Maize crops are very susceptible to pests and various types of pathogens, such as viruses and fungi. One of the viruses generally infect maize crops is Cucumber mosaic virus (CMV). CMV is a virus species which belongs to family Bromoviridae. Maize cultivars Bima-4, Bisma, Lamuru, and Sukmaraga are resistant to grain of disease, leaf spots and leaf carats. Maize cultivar Provit-A has a level of tolerance to a variety of pests, meanwhile cultivar Talenta is resistant to downy mildew, carats and leaf blights. Research on resistance to CMV in Maize cultivars Bima-4, Bisma, Lamuru, and Sukmaraga on the field have been determinedbyscoring, but there is no informationon detection of resistance gene against CMV in hybrid�s maize (cultivars Bima-4, Bisma, Lamuru, and Sukmaraga) and commercial�s maize (cultivars Provit-A and Talenta). The aim of this research was to detect resistance gene against CMV in hybrid�s maize (cultivars Bima-4, Bisma, Lamuru, and Sukmaraga) and commercial�s maize (cultivars Provit-A and Talenta). Resistance gene against CMV on cultivars Bima-4, Bisma, Lamuru, Sukmaraga, Provit-A and Talenta was detectedusing Polymerase Chain Reaction (PCR) and analyzed with electrophoresis. The results showed resistance gene against CMV is not found in the six cultivars using MRGA-f1 on 438 bp, that was used inMelon. Hence it could be revealed that maize has different resistance mechanism against CMV.