ANALISIS KONSENTRASI DNA VIRUS DAN MUTASI BASAL CORE PROMOTER PADA PASIEN DENGAN HEPATITIS B KRONIS DAN PENYAKIT HATI TAHAP LANJUT DI RSUP Dr. SARDJITO
Background: Hepatitis B virus (HBV) infection was exceptionally prevalent in Southeast Asia, including Indonesia. Acute viral infection might resolve completely, as well as develop into chronic infection and advanced liver diseases, all of them marked by serological changes observed in the patient....
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| 格式: | Theses and Dissertations NonPeerReviewed |
| 出版: |
[Yogyakarta] : Universitas Gadjah Mada
2014
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| 在線閱讀: | https://repository.ugm.ac.id/128787/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69154 |
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| 總結: | Background: Hepatitis B virus (HBV) infection was
exceptionally prevalent in Southeast Asia, including
Indonesia. Acute viral infection might resolve
completely, as well as develop into chronic infection
and advanced liver diseases, all of them marked by
serological changes observed in the patient.
Improvement of patient�s serological status without any
clinical improvement was suggested to be related to
various viral genetic mutations, such as double
mutation (A1762T/G1764A) in basal core promoter (BCP)
region, which might also be related to enhanced viral
replicative potential and DNA concentration.
Objectives: The aim of this study was to determine the
prevalence of basal core promoter mutation in patients
diagnosed with chronic viral infection and advanced
liver diseases, as well as significance of relationship
between DNA concentration and mutation profiles in
forementioned populations.
Methods: This study employed cross-sectional method
performed in Dr. Sardjito General Hospital and Medical
Faculty of Gadjah Mada University, Yogyakarta. Serum
samples were taken from peripheral blood of patients
with positive HbsAg. DNA extraction was then performed,
followed by polymerase chain reaction (PCR) for
amplification. Electrophoresis was also performed to
determine mutation profile.
Result: Mutations in basal core promoter was prevalent
in observed groups (41.25%). In addition, there was
significant correlation between A1762T/G1764A double
mutation and viral DNA concentration in studied
population (p value = 0.007).
Conclusion: Prevalence of A1762T/G1764A double mutation
in basal core promoter region was relatively high among
studied population. In addition, the correlation of
viral DNA concentration and mutations in basal core
promoter might be used in determination of liver
disease progression. |
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