ANALISIS KONSENTRASI DNA VIRUS DAN MUTASI BASAL CORE PROMOTER PADA PASIEN DENGAN HEPATITIS B KRONIS DAN PENYAKIT HATI TAHAP LANJUT DI RSUP Dr. SARDJITO

• Main Authors: , KATARINA DIAN ANDINI, , Dr. Didik Setyo Heriyanto, Ph.D, Sp.PA
• Format: Theses and Dissertations NonPeerReviewed
• Published: [Yogyakarta] : Universitas Gadjah Mada 2014
• Subjects: ETD
• Online Access: https://repository.ugm.ac.id/128787/; http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=69154

Background: Hepatitis B virus (HBV) infection was exceptionally prevalent in Southeast Asia, including Indonesia. Acute viral infection might resolve completely, as well as develop into chronic infection and advanced liver diseases, all of them marked by serological changes observed in the patient. Improvement of patient�s serological status without any clinical improvement was suggested to be related to various viral genetic mutations, such as double mutation (A1762T/G1764A) in basal core promoter (BCP) region, which might also be related to enhanced viral replicative potential and DNA concentration. Objectives: The aim of this study was to determine the prevalence of basal core promoter mutation in patients diagnosed with chronic viral infection and advanced liver diseases, as well as significance of relationship between DNA concentration and mutation profiles in forementioned populations. Methods: This study employed cross-sectional method performed in Dr. Sardjito General Hospital and Medical Faculty of Gadjah Mada University, Yogyakarta. Serum samples were taken from peripheral blood of patients with positive HbsAg. DNA extraction was then performed, followed by polymerase chain reaction (PCR) for amplification. Electrophoresis was also performed to determine mutation profile. Result: Mutations in basal core promoter was prevalent in observed groups (41.25%). In addition, there was significant correlation between A1762T/G1764A double mutation and viral DNA concentration in studied population (p value = 0.007). Conclusion: Prevalence of A1762T/G1764A double mutation in basal core promoter region was relatively high among studied population. In addition, the correlation of viral DNA concentration and mutations in basal core promoter might be used in determination of liver disease progression.